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Home / Issues / № 1, 2011

ABOUT METHOD OF INCREASING BIOCHEMICAL ACTIVITY OF LACTOSE FERMENTATIVE YEAST
Olmoeva V.D., Khamnaeva N.I.
Lactose fermentative biological objects, being technically useful microflora, should have technological charecteristics. In this regard search of new methods of their cultivation, increasing the efficiency of fermentation process is very important.

Many researchers have been studying influence of medium formulation on microorganisms´ growth. So, Zalashko got the best results of cultivating strain S. Fragilis on milk whey adding 0,75 % of ammonia sulphate, 0,5 % of monosubstituted potassium phosphate and 0,1 % of yeastrel (37,05). Wasserman et al. got the maximum crop by adding 0,5 % of ammonia sulphate, 0,5 % of monosubstituted potassium phosphate and 0,1 % of yeastrel to milk serum [1].

According to Bannikova [3] cultivation of yeast in temperature close to upper limits of comfort temperature of growth usually stimulates their growth.

Abramov S.A., Kostenko S.T, Khalilova E.A., Islamova F.I., Omarov M.M. [3] grew yeast on nutritional medium where as mineral and organic nutrition geothermal water of non phenolic class was used, watered down up to mineralization 4,0-4,2 g/l. The method allows decreasing spending deficit components of mineral and organic nutrition.

Abramov S.A., Kotenko S.T., Dalgatova B.I., Efendieva D.A., Khalilova E.A. [4] made nutritious medium for growing bakery yeast in the following way: into molasses watered down up to mineralization 2,0-2,4 gl we add geothermal water till 9,5 % carbohydrate status and then diammonium phosphate. And medium ratio is (gl): molasses 160-180, diammonium phosphate 1,0-1,5, geothermal water - the rest.

Polandova R.D. and Bykovchenko T.V [5] worked out a method of getting yeast biomass, providing yeast baking strength improvement up to 20 %, fermentative activity up to 8,0 %, yeast cells increase up to 11,5 %. The method includes preparing brew out of flour and water, its saccharification, fermentation with thermophile lactic-acid bacteria, adding mineral salts К2HPO4 and (NH4)2SO4 and growing yeast on the obtained nutritious medium with extraction of liquid yeast and giving nutritious medium every 3-4 hours.

Thus using microbic cell´s ability to change its metabolism with environmental factors changes it becomes real to manage the yield of biomass and base products.

Important object is to increase biomass of lactose fermentative yeast yield. With that it makes sense to use peptone as source of nitrogenous matter, yeastrel as bioactive substance, geothermal water as mineral compound.

References

  1. Physiological regulation of yeast metabolism/ under editorship of M.V. Zalashko // Навука i тэхнiка. - 1991. - 332 p.
  2. Bannikova L.A., Koroleva N.S., Semenikhina V.F. Microbiological bases of milk production. - M.: Agropromizdat, 1987. - 400 p.
  3. Patent 2151795 МПК 7 C12N1/16, C12N1/18 Method of getting dried yeast / Abramov S.A., Kotenko S.T., Dalgatova B.I., Efendieva D.A., Khalilova E.A., Islamova F.I., Omarov M.M.
  4. Patent 2084519 МПК6 C12N1/18 18 Method of getting nutrious medium for growing bakery yeast / Abramov S.A., Kotenko S.T., Dalgatova B.I., Efendieva D.A., Khalilova E.A.
  5. Patent 2326937 IPC C12N1/18 (2006.01) Method of preparing liquid yeast / Polandova R.D., Bykovchenko T.V

The work was submitted to the international science conference «Contemporary science technologies», (Egypt), 21-28 February, 2010. Came to the editorial office on 12.01.2010.


Bibliographic reference

Olmoeva V.D., Khamnaeva N.I. ABOUT METHOD OF INCREASING BIOCHEMICAL ACTIVITY OF LACTOSE FERMENTATIVE YEAST. International Journal Of Applied And Fundamental Research. – 2011. – № 1 –
URL: www.science-sd.com/387-23511 (29.03.2024).