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Asgarov S. (Azerbaijan), Alakbarov M. (Azerbaijan), Aliev Z. (Azerbaijan), Babayev N. (Uzbekistan), Chiladze G. (Georgia), Datskovsky I. (Israel), Garbuz I. (Moldova), Gleizer S. (Germany), Ershina A. (Kazakhstan), Kobzev D. (Switzerland), Kohl O. (Germany), Ktshanyan M. (Armenia), Lande D. (Ukraine), Ledvanov M. (Russia), Makats V. (Ukraine), Miletic L. (Serbia), Moskovkin V. (Ukraine), Murzagaliyeva A. (Kazakhstan), Novikov A. (Ukraine), Rahimov R. (Uzbekistan), Romanchuk A. (Ukraine), Shamshiev B. (Kyrgyzstan), Usheva M. (Bulgaria), Vasileva M. (Bulgar).

The practically healthy people´s at the age in the range from 3 up to 45 years microbial flora has been studied and thoroughly investigated by us. The microscopic and the culture methods use have been shown, that the protozoa infection has been made up 2,73 % (e.g. 3 persons) just in this group, and this, moreover, is, considerably, being lowered the people´s with the gastrointestinal tract pathologies analogous index. So, in all these cases, the protozoa finding has been accompanied with the bowels microflora disbiotic changes, having, at the same time, the various degree of the manifestation.
The «normal» microflora notion has already been defined, as the large intestine bacterial dissemination average physiological value, having taken into consideration the received final results´ oscillations.
All these investigations have been carried out, having defined the qualitative and the quantitative compositions of the bacterial communities with the following space structure identification and also the ecological groups´ hierarchy.
The received final data analysis has been permitted to be identified the 390 microorganisms strain and also to be related them to the 11 deliveries.
The bowels microflora composition study and the thoroughly investigation had already been shown, that its composition was enough diverse and suffice various (table 1). The obligate - anaerobic flora analysis was been shown, that the bowels´ colonization density by the obligate - anaerobic bacteria had been presented by the representatives´ high level concentrations, having been studied and thoroughly investigated taxa. So, the bacteroides´ values oscillations have been from 108 up tо 1010 QEU/gr. The clostridia´s dissemination lower bound has not been exceeded 105 QEU/gr. Thus, the healthy man´s average colonization density by the obligate - anaerobic microorganisms has been defined, as lgr. 10,30,7 QEU/gr.
Table 1. The Large Intestine´s Microflora Main Representatives
Microorganisms |
Abs. |
% |
1gr. QEU/gr., Mm |
Lactobacteria |
80 |
100,0 |
8,80,6 |
Bifidus bacteria |
80 |
100,0 |
9,30,1 |
Escherichia |
80 |
100,0 |
7,40,3 |
Bacteroides |
52 |
65,0 |
10,30,1 |
Enterococcus |
14 |
17,5 |
4,70,2 |
Enterobacteria (totally) |
17 |
21,3 |
3,70,2 |
Protei |
8 |
10,0 |
3,50,1 |
Клебсиеллы |
6 |
7,5 |
3,40,7 |
Enterobacter |
3 |
3,7 |
3,10,5 |
Staphylococci |
16 |
20,0 |
4,10,4 |
Candida Fungi |
22 |
27,5 |
3,30,1 |
Clostridia |
5 |
6,2 |
6,00,8 |
So, the singled out microorganisms identification was shown, that the bacteroides were being occupied the dominating situation among the anaerobic flora, which were being made up the most numerous bacteria group, as by the character frequency, well as by their colonization density. Thus, the healthy people´s average bacteroides´ content just in the bowels has been equaled to 10,30,1 QEU/gr., and they have been discovered at the 65,0 % examined persons. The bacteroides - are the Gram - positive anaerobic bacilli, which are not being formed the spores; they give the following growth, just in the form of the whitish or the grayish colonies with the smooth edges, having had the 3 - 6 mm [1] just on the solid and nutrient medium.
By the authors´ majority data, the clostridia are the Gram - positive, spore - forming, frequently movable, bacilliform bacteria, and the obligate anaerobes [4]. They are being related to the bowels´ normal microflora optional part. But they are being found seldom, and, moreover, their quantity will not have to be exceeded 107 QEU/gr. just in the normal biocoenosis [2,5]. The clostridia have been found just at 5 persons, that has been made up 6,25 % in the carried out studies and thoroughly investigations, for all this, the dissemination density has been varied in the range from 105 up to 107 microbial bodies, having had the average value, which is equal to lgr. 6,0 0,8 QEU/gr.
The aerobic microflora analysis has been shown, that the microorganisms´ abundance is being dominated just in the healthy people´s bowels. The biocoenosis´ constant representatives have been the lactobacteria (e.g. 100,0 %), for all this, all these microbes have been found just in the titres in the range of 107 - 1010 QEU/gr. at the examined majority.
The bifidus bacteria have also, frequently, been discovered just in the microflora (e.g. 100,0 %), for all this, the dissemination average arithmetic value has been rather considerable (e.g. lgr. 9,30,1 QEU/gr.).
The considerable physiological role is being belonged to the bacteria bowels´ group among the large intestine aerobic flora. The study and the thoroughly escherichia qualitative and the quantitative compositions investigation are being presented the quite reasonable ones for us. It has, moreover, been shown in this work, that the E. coli have been found at all the examined ones (e.g. 100 %) with the values fluctuations just in the range of from the 200 mln. up to 1 bln. microbial bodies in 1 gr. faeces, for all this, the dissemination value was equal to lg 7,40,3 QEU/gr.
The opportunistic pathogen enterobacteria (e.g. 21,25 %) have been sowed, quite often, just from the healthy people´s faeces. The high microbial number has been fixed just for the protei - lgr. 3,50,1 QEU/gr. and also the клебсиелл - lgr. 3,40,7 QEU/gr., the enterobacteria (lgr. 3,10,5 QEU/gr.) have been discovered the less dissemination.
The staphylococci and the fungi sort of Candida have been met, enough frequently, among the opportunistic pathogen microorganisms. The representatives sort of Staphylococcus have been found at the 20,0 % persons, for all this the S. epidermidis (e.g. 15,0 %) has been sowed much more frequently, the S. aureus (e.g. 3,75 %) - considerably much infrequent, and 1,25 % has been fallen for the part of the rest sorts and types.
The yeast - like fungi sort of Candida (e.g. 27,5 %) have been registered, fairly often, at the healthy people´s microflora. However, the dissemination has been rather insignificant by all these microorganisms, and the average arithmetic value has been made up lgr. 3,30,1 QEU/gr..
Thus, all these carried out studies and thoroughly investigations have been shown, that the healthy people´s large intestine microflora is usually being characterized by the aerobial microorganisms´ dominance. It should be noted, that this microorganism is quite able to be used, as the indicator one, at the microflora carried out study and thoroughly investigation, which is, as in the quite norm, well as at the pathology.
References
- Grigorjev P.Ya., Yakovenko A.P. "The Intestinal Microflora Normal Composition Abnormality, the Clinical Significance and the Therapeutics´ Challenges". - Moscow. - 2000 - p. 16;
- Dorofeev A.A., Nesvizhsky Yu.V., "The Large Intestine Microbiocenosis at the Non - Specific Ulcerative Colitis Patients" // "The Epidemiology and the Infectious Diseases". - 1997, № 2. - p.p. 26 - 29;
- Pokrovsky V. E., Pozdeev O.K., "The Medical Microbiology". - Moscow, 1999, - p. 1184;
- Steinier R., Edelberg E., Engrem J., "The Microbes´ World" / R. Steinier [and etc.] / The Translation from English. - М.: The World. - 1979. - vol.2. - p. 320;
- Gibson T.M., Klary E.H., Norton B.B., Temros J.H., Cassidy G.R., Praston V.D., "The Microbiota of the Gastroitestinal Tract" // "Gastroenterology". - 1994. - V.51, №5. - p.p. 808 - 815.
The work was submitted to international scientific conference «General practitioner», September 6-13, 2009, Italy (Rome, Florence), came to the editorial office on 09.07.2009.
Ilyina N.A., Karpeeva E.A. THE PRACTICALLY HEALTHY PEOPLE’S LARGE INTESTINE MICROBIAL FLORA COMPOSITION. International Journal Of Applied And Fundamental Research. – 2009. – № 2 –
URL: www.science-sd.com/385-23409 (08.06.2025).